Vibrio cholerae
Killed Oral Vaccine against Cholera
In a series of Phase 1 clinical studies funded by a Programme Grant from the Wellcome Trust Prof David Lewis, Professor Griffin, Prof Dougan and Dr Luiz Castello-Branco determined in detail the kinetics of the T and B cell responses of humans to oral cholera whole-cell / B subunit vaccine developed by SBL Vaccin Sweden. These studies are published and provided the model for subsequent oral vaccine studies.
In addition, Dr Lewis and Prof Griffin described the effect of infection with HIV and the onset of AIDS on the immune responses to the oral cholera vaccine. These studies laid the foundation for further studies in Brazil by Dr Luiz Castello-Branco, Dr Ortigao, Prof Griffin, Dr R Shattock, Dr Lewis and others on the effect of oral vaccination on circulating HIV virus.
Publications
Douce G, Giannelli V, Pizza M, Lewis DJM, Everest P, Rappuoli R, Dougan. Genetically detoxified mutants of heat-labile toxin from Escherichia coli are able to act as oral adjuvants. Infection & Immunity 1999; 67 (9): 4400-4406.
Ortigão-de-Sampaio MB, Shattock RJ, Hayes P, Griffin GE, Linhares-Carvalho MI, Ponce de Leon A, Lewis DJM, Castello-Branco LRR. Increase in plasma viral load after oral cholera immunisation of HIV-infected subjects. AIDS 1998; 12: 145-150.
Castello-Branco LR, Lewis DJ, Ortigo-de-Sampaio MB, Griffin GE. Gastrointestinal immune responses in HIV infected subjects. Mem Inst Oswaldo Cruz 1996;91(3):363-6.
Castello-Branco LRR, Griffin GE, Dougan G, Lewis DJM. A method to screen T lymphocyte epitopes after oral immunisation of humans: application to cholera toxin B subunit. Vaccine 1995; 13 (9): 817-820.
Lewis DJM, Gilks CF, Ojoo S Castello-Branco LRR, Dougan G, Evans, MR, McDermott S, Griffin GE. The immune response following oral administration of cholera toxin B subunit to HIV-1 infected United Kingdom and Kenyan subjects. AIDS 1994; 8(6): 779-85.
Castello-Branco LRR, Griffin GE, Poulton TA, Dougan G, Lewis DJM. Characterisation of the circulating T cell response after oral immunisation of human volunteers with Cholera Toxin B subunit. Vaccine 1994; 12 (1): 65-72.
Lewis DJM, Castello-Branco LRR, Novotny P, Dougan G, Poulton TA, Griffin GE. Circulating cellular immune response to oral immunisation of humans with cholera toxin B subunit. Vaccine 1993; 11(2): 119-121.
Lewis DJM, Novotny P, Dougan G, Griffin GE. The early cellular and humoral immune response to primary and booster oral immunisation with cholera toxin B subunit. European Journal of Immunology 1991; 21: 2087-2094.
